Published: 30 December, 2016 | Volume 1 - Issue 1 | Pages:
Figure 1:
A. Schematic drawing of the perfusion system. The overflow head-tank provided a steady flow to the parallel-plate flow chamber. The filtration rate measurement cell on the abluminal side of the culture was filled with the same fluid as the perfusate. B. Water filtration rate for the permeable group with concentration of DiIox-LDL varied from 1 to 200 μg/ml. C. Wall concentration of DiI-ox-LDL in permeable group was assessed by measuring the fluorescence intensity of DiI-ox-LDL using a confocal laser microscope. All data were normalized with the bulk concentration, c0. D. Effect of ox-LDL on EC proliferation in endothelial cells was assessed by MTT assay. E. Trypan blue staining data in ECs cultured on permeable or non-permeable membranes and treated with ox-LDL (1 to 200 μg/ml). EC suspensions were exposed to perfusion pressure of 100 mmHg for 24 h. The results are expressed as means ± SD (n= 6). *P< 0.05 between permeable and non-permeable groups.
Read Full Article HTML DOI: 10.29328/journal.jccm.1001003 Cite this Article Read Full Article PDF
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