Abstract

Research Article

Are S-Klotho’s Maximal concentrations dependent on Exercise Intensity and Time in young adult males?

Moran Sciamama Saghiv*, David Ben-Sira, Ehud Goldhammer and Michael Sagiv

Published: 08 March, 2019 | Volume 4 - Issue 1 | Pages: 006-011

The purpose of the present study was to define the period of time in which aerobic training does not increase further serum S-Klotho levels in untrained young adult males, and to examine the relation between plasma S-Klotho concentration and maximal oxygen uptake (VO2max).

Methods: Sixty (60) untrained subjects (27.05±1.1 years) were divided into 2 groups, both exercised six months 4×wk-1 for the duration of 45 min×session. One group (LTI) exercised below the anaerobic threshold at 40-50% of VO2max, while the second group (HTI) worked above the anaerobic threshold at 65-70% of VO2max. Testing sessions were performed at 0, 2, 4, and 6 months. Blood samples were drawn after overnight fasting; S-Klotho was analyzed using an ELISA kit.

Results: Following 2 and 4 months, significant (p≤0.05) increases were noted in the HTI group, at the fourth testing session, S-Klotho leveled off. In the LTI group, S-Klotho remained almost unchanged. Findings of the present study, support emerging evidence suggesting that a relation between plasma S-Klotho concentration and VO2max exists.

Conclusion: Data suggest that increases in S-Klotho is tidally associated with VO2max levels. In addition, the S-Klotho increase levels-off following 4 months of aerobic training. Exercising below the anaerobic threshold does not increase VO2max and thus, does not increase S-Klotho.

 

Read Full Article HTML DOI: 10.29328/journal.jccm.1001033 Cite this Article Read Full Article PDF

Keywords:

Anti-aging; Aerobic training; Untrained individuals; Maximal oxygen uptake; ELISA kit; ROS; Nitric acid

References

  1. Maekawa Y, Ohishi M, Ikushima M, Yamamoto K, Yasuda O, et al. Klotho Protein Diminishes Endothelial Apoptosis and Senescence via a Mitogen-Activated Kinase Pathway. Geriatr Gerontol Int. 2011; 11: 510-516. Ref.: https://goo.gl/T2kFua
  2. Kim JH, Hwang KH, Park KS, Kong ID, Cha SK. Biological Role of Anti-Aging Protein Klotho. J. Lifestyle Med. 2015; 5: 1-6. Ref.: https://goo.gl/3ywkYX
  3. Navarro-González JF, Donate-Correa J, Muros de Fuentes M, Pérez-Hernández H, Martínez-Sanz R, et al. Reduced Klotho is Associated with the Presence and Severity of Coronary Artery Disease. Heart. 2014; 100: 34-40. Ref.: https://goo.gl/8mRBnH
  4. Kingsley D. Aging and Health Care Costs: Narrative versus Reality. Poverty & Public Policy. 2015; 7:3-21. Ref.: https://goo.gl/uZHt4t
  5. Martín-Núñez E, Donate-Correa J, Muros-de-Fuentes M, Mora-Fernández C, Navarro-González JF. Implications of Klotho in Vascular Health and Disease. World J Cardiol. 2014; 6: 1262-1269. Ref.: https://goo.gl/8ruuoV
  6. Saghiv MS, Sira DB, Goldhammer E, Sagiv M. The Effects of Aerobic and Anaerobic Exercises on Circulating Soluble-Klotho and IGF-I in Young and Elderly Adults and in CAD Patients. J Circ Biomark. 2017; 6:1849454417733388. Ref.: https://goo.gl/VKkH6z
  7. Saghiv M. Effects of Aerobic Exercise Training on S-Klotho in Young and Elderly. JJ Physiology. 2015; 1: 001. Ref.: https://goo.gl/8W69JV
  8. Behenke AR, Wilmore J. Evaluation and regulation of Body Build and Composition. Englewood Cliffs, N.J. Prentile Hall, Inc. 1974;.
  9. American College of Sports Medicine. ACSM's Guidelines for Exercise Testing and Prescription, 10th edition, Philadelphia, PA: Lippincott Williams & Wilkins; 2014; 145-147 and 165-199. Ref.: https://goo.gl/ZVQKaC
  10. Yamazaki Y, Imura A, Urakawa I, Shimada T, Murakami J, et al. Establishment of Sandwich ELISA for Soluble Alpha-Klotho Measurement: Age-Dependent Change of Soluble Alpha-Klotho Levels in Healthy Subjects. Biochem Biophys Res Commun 2010; 398: 513–518. Ref.: https://goo.gl/81XzNR
  11. Pedersen L, Pedersen SM, Brasen CL, Rasmussen LM. Soluble Serum Klotho Levels in Healthy Subjects. Comparison of Two Different Immunoassays. Clin Biochem 2013; 46:1079–1083. Ref.: https://goo.gl/yjgXPg
  12. Heijboer AC, Blankenstein MA, Hoenderop J, de Borst MH, Vervloet MG, et al. Laboratory Aspects of Circulating Alpha-Klotho. Nephrol Dial Transplan. 2013; 28: 2283-2287. Ref.: https://goo.gl/RymBJD
  13. McArdle A, Pattwell D, Vasilaki A, Griffiths RD, Jackson MJ. Contractile Activity-Induced Oxidative Stress: Cellular Origin and Adaptive Responses. Am J Physiol Cell Physiol. 2001; 280: C621-627. Ref.: https://goo.gl/rHtB8f
  14. McArdle A, Pollock N, Staunton CA, Jackson MJ. Aberrant Redox Signalling and Stress Response in Age-Related Muscle Decline: Role in Inter and Intra-Cellular Signalling. Free Radic Biol Med. 2019; 132: 50-57. Ref.: https://goo.gl/7x7EuQ
  15. Powers KS, Jackson JM. Exercise-Induced Oxidative Stress: Cellular Mechanisms and Impact on Muscle Force Production. Physiol Rev. 2008; 88: 1243-1276. Ref.: https://goo.gl/7UtBHB
  16. Powers KS, Nelson WB, Hudson BM. Exercise-Induced Oxidative Stress in Humans: Cause and Consequences. Free Radical Biology and Medicine. 2011; 51: 942-950. Ref.: https://goo.gl/ruxq6D
  17. Carracedo J, Buendía P, Merino A, Madueño JA, Peralbo E, et al. Klotho Modulates the Stress Response in Human Senescent Endothelial Cells. Mech Ageing Dev. 2012; 133: 647-654. https://goo.gl/wnb5Sr
  18. Avin KG, Coen PM, Huang W, Stolz DB, Sowa GA, et al. Skeletal Muscle as a Regulator of the Longevity Protein, Klotho Front. Physiol. 2014; 5: 189. Ref.: https://goo.gl/oEawDv
  19. American College of Sports Medicine, Chodzko-Zajko WJ, Proctor DN, Fiatarone Singh MA, Minson CT, et al. American College of Sports Medicine Position Stand. Exercise and Physical Activity for Older Adults. Med Sci Sports Exerc. 2009; 41: 1510-1530. Ref.: https://goo.gl/NcmTcx
  20. Carter H, Jones AM, Doust JH. Effect of Six weeks of Endurance Training on the Lactate Minimum Speed. J Sports Sci. 1999; 17: 957-967. Ref.: https://goo.gl/hKSVXv
  21. Weltman A. The Blood Lactate Response to Exercise. Champaign, IL: Human Kinetics. 1995; Ref.: https://goo.gl/RjXWat
  22. Yamamoto M, Clark JD, Pastor JV, Gurnani P, Nandi A, et al. Regulation of Oxidative Stress by the Anti-aging Hormone Klotho. J Biol Chem. 2005; 280: 38029-38034. Ref.: https://goo.gl/h6tqix

Figures:

Figure 1

Figure 1

Figure 1

Figure 2

Similar Articles

Recently Viewed

Read More

Most Viewed

Read More

Help ?